如果您不介意斜杠被切换，则可以[ab]使用Uri：

Uri file = new Uri(@"c:\foo\bar\blop\blap.txt");
// Must end in a slash to indicate folder
Uri folder = new Uri(@"c:\foo\bar\");
string relativePath = 
Uri.UnescapeDataString(
    folder.MakeRelativeUri(file)
        .ToString()
        .Replace('/', Path.DirectorySeparatorChar)
    );

作为功​​能/方法：

string GetRelativePath(string filespec, string folder)
{
    Uri pathUri = new Uri(filespec);
    // Folders must end in a slash
    if (!folder.EndsWith(Path.DirectorySeparatorChar.ToString()))
    {
        folder += Path.DirectorySeparatorChar;
    }
    Uri folderUri = new Uri(folder);
    return Uri.UnescapeDataString(folderUri.MakeRelativeUri(pathUri).ToString().Replace('/', Path.DirectorySeparatorChar));
}