现代ES6方式 （异步/等待）

const toBase64 = file => new Promise((resolve, reject) => {
    const reader = new FileReader();
    reader.readAsDataURL(file);
    reader.onload = () => resolve(reader.result);
    reader.onerror = error => reject(error);
});

async function Main() {
   const file = document.querySelector('#myfile').files[0];
   console.log(await toBase64(file));
}

Main();

UPD：

如果您想捕捉错误

async function Main() {
   const file = document.querySelector('#myfile').files[0];
   const result = await toBase64(file).catch(e => Error(e));
   if(result instanceof Error) {
      console.log('Error: ', result.message);
      return;
   }
   //...
}